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Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, rabbit IgG
AS10 1195 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)
- Product Info
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Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Liquid Quantity: 1 mg Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB) - Additional Information
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Additional information: This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human and rabbit IgG based on immunoelectrophoresis.
Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. - Background
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Background: Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,rabbit IgG and are affinity purified using solid phase sheep IgG. - Protocols
- Antibody protocols
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Accessories
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