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Donkey anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG
AS10 1115 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L) except Rat Ig1
- Product Info
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Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: The optimal working dilution should be determined by the investigator - Additional Information
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Additional information: This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis, except rat Ig1.
Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep by immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
- Background
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Background: Donkey anti-rat IgG (H&L),HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays, except rat Ig1. Antibody is adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG, and affinity purified using solid phase rat IgG.
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Accessories
AS10 1258 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rat IgG (H&L)
AS10 1218 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L)
AS10 947 |Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L)