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Donkey anti-Rat IgG (H&L), FITC conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG
AS10 1282 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rat IgG (H&L)
- Product Info
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Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Liquid Quantity: 1 mg Storage: Store at 2-8°C. Shelf life of this product is one year from date of receipt. Recommended dilution: 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt) - Additional Information
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Additional information: This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
- Background
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Background: Donkey anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse, rabbi,sheep IgG and affinity purified using solid phase rat IgG. - Product Citations
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Selected references: Zhu et al. (2024). The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis. Plant Cell . 2024 Oct 29:koae292. doi: 10.1093/plcell/koae292.
Wijnker et al. (2019). The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12534-12539. doi: 10.1073/pnas.1820753116. - Protocols
- Antibody protocols
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Accessories
AS10 1214 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L)
AS10 1258 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rat IgG (H&L)
AS10 1138 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L)
AS10 1007 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L)