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Donkey anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
AS10 1008 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L)
- Product Info
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Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: The optimal working dilution should be determined by the investigator - Additional Information
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Additional information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
- Background
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Background: Donkey anti-rabbit IgG (H&L) is a secondary antibody, HRP conjugated, which binds to all rabbit IgGs in immunological assays. Antibody is specially adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG. Antibodies are affinity purified using solid phase rabbit IgG. - Product Citations
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Selected references: Petersen and Andersen (2014). Simultaneous isolation of mRNA and native protein from minute samples of cells. Biotechniques. 2014 May 1;56(5):229-37. doi: 10.2144/000114165. eCollection 2014. - Protocols
- Antibody protocols
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Accessories
AS10 1269 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rabbit IgG (H&L)
AS10 1299 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rabbit IgG (H&L)
AS10 842 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (heavy and light chains - H&L)
