Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG

Name: Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
SKU: AS10 1019
Price: 246 EUR
Availability: InStock

Product no: AS10 1019

AS10 1019 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L)

246 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Host:	Donkey
Clonality:	Polyclonal
Purity:	Immunogen affinity purified IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
Storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Recommended dilution:	The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)

Additional Information

Additional information:

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Background

Background:

Donkey anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG, and affinity purified using solid phase rabbit IgG.

Protocols
Antibody protocols
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