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Donkey anti-Rabbit IgG (H&L), HRP conjugated
AS10 845 | Clonality: Polyclonal | Host: doneky | Reactivity: Rabbit IgG (H&L)
- Product Info
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Immunogen: Purified Rabbit IgG
Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified donkey IgG. Format: Lyophilized Quantity: 1 mg Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Rabbit IgG (H&L) Predicted reactivity: Rabbit IgG (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Additional information (application): This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
- Background
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Background: Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
- Product Citations
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Selected references: Cook et al. (2024). Chloroplast phosphatases LPPγ and LPPε1 facilitate conversion of extraplastidic phospholipids to galactolipids. Plant Physiol. 2024 May 31;195(2):1506-1520. doi: 10.1093/plphys/kiae100. - Protocols
- Antibody protocols
- Reviews:
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Accessories
AS10 1127 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L)
AS10 1269 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rabbit IgG (H&L)
AS10 1168 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L)