Donkey anti-Rabbit IgG (H&L), HRP conjugated

Product no: AS10 845

AS10 845 | Clonality: Polyclonal  |  Host: doneky  | Reactivity: Rabbit IgG (H&L)

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  • Product Info
  • Immunogen:

    Purified Rabbit IgG

    Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified donkey IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: The optimal working dilution should be determined by the investigator
  • Reactivity
  • Confirmed reactivity: Rabbit IgG (H&L)
    Predicted reactivity: Rabbit IgG (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

    0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

    Additional information (application):

    This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

    No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

  • Background
  • Background:

    Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

  • Product Citations
  • Selected references: Cook et al. (2024). Chloroplast phosphatases LPPγ and LPPε1 facilitate conversion of extraplastidic phospholipids to galactolipids. Plant Physiol. 2024 May 31;195(2):1506-1520. doi: 10.1093/plphys/kiae100.  
  • Protocols
  • Antibody protocols
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