Anti-CURT1B | Curvature thylakoid 1B

Samples with 2 µg of total chlorophyll from Arabidopsis thaliana thylakoid fractions were denatured with 250mM DTT at 95°C 5 min, extracts were separated on 12% SDS-PAGE and blotted 7 min to PVDF using semi-dry transfer. Blot was incubated in the primary antibody (and 5% milk) at a dilution of 1: 2000 ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed with Agrisera chemiluminescent detection reagent, ECLSuperBright. Exposure time was 5 seconds.

Courtesy of Guillem Borràs I Gas , Pribil Lab, University of Copenhagen, Danmark

Curvature thylakoid 1B (CURT1B) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D.  It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.