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Anti-Cu/ZnSOD | Cu/Zn superoxide dismutase
AS10 652 | Clonality: Polyclonal | Host: Chicken | Reactivity: L. longiflorum, O. europaea, O. sativa, S. lycopersicum, S. oleracea
- Product Info
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Immunogen: 15 amino acids synthetic peptide designed from olive pollen cytosolic Cu/Zn cDNA consensus sequences (GenBank Accessions EU250757 to EU250797), UniProt Q8L5E0
Host: Chicken Clonality: Polyclonal Purity: Purified, total IgY in 10 mM Tris, 150 mM NaCl, 0.02% sodium azide. Format: Liquid Quantity: 100 µl Storage: Store at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1500 with Alexa 488 (WB) Expected | apparent MW: 15.3 | 16 kDa (Olea europaea L.)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana (leaves), Lilium longiflorum, Olea europaea (pollen), Oryza sativa, Spinacia oleracea (leaves), Solanum lycopersicum, Zea mays Predicted reactivity: Ananas ananas, Betula pendula, Camellia sinensis, Codonis lanceolata, Cucurbita ficifolia, Helianthus sp., Hordeum vulgare, Lycopersicum esculentum, Plantago major, Populus trichocarpa, Solanum nigrum, Solanum tuberosum, Solidago sp., Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: Helianthus annuus , Nicotiana tabacum, Marchantia polymorpha, Musa acuminata, Vicia faba
- Application Examples
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Application example
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25 µg of total protein from Olea europaea pollen were separated on 12% acrylamide gels and blotted onto PVDF. Membranes were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Cu/Zn SOD (AS10 652, 1:1500, overnight, 4º C) and secondary antibody anti-chicken IgG conjugated to Alexa 488 (1:2000, 1 h) (Molecular Probes, Invitrogen). Signal was detected with a PharosSX fluorescence scanner (Bio-Rad).
The antibody recognizes a protein of c.a. 16 kDa. and another band likely corresponding to the protein dimer.Courtesy Dr. Juan de Dios Alche, CSIC, Spain
- Additional Information
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Additional information: Total IgY concentration is 2.3 mg/ml.
Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.
Additional information (application): Note: Antibody recognizes two to three isoforms of Cu/Zn SOD in olive pollen depending on the olive cultivar - Background
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Background: Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
- Product Citations
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Selected references: Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.
Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Alché et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Susanne Braun | 2017-01-24We have used PC antibody with protein extrac from Hordeum vulgare.We got a special bandLoad per well: 15µg total Protein, 14% SDS-GelMembrane: NC von Roth, HP40.1Blocken: 5% milk in TBST, 2h, RTCU/ZnSOD ( AS10 652 ): 1:1000 in 2% milk, 1 x TBST, over night, 4°CSecondary antibody : Rabbit anti goat IgG AS 09605 1:50000 in 2% milk / 1 x TBST 1h RT Developing reagent: ECL ( GE Healthcare )1:1:2Exposure time: 1sec.Alicja Buczynska | 2014-05-08I used this antybody for Cu/ZnSOD immunodetection analysis in the leaves of three grasses: Echinochloa crus-galli, Digitaria sanguinalis and Zea mays plants (all NADP-ME subtype). For each treatment 15 µg of total proteins extract from the leaves were loaded on SDS-PAGE (12% acrylamide) and immunodetected with anti-Cu/ZnSOD (Agrisera). The highest signal was observed in Echinochloa crus-galli, Digitaria sanguinalis, and the weakest signal was noted in Zea mays in different irradiation of the light.
Accessories
AS06 125 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. maritima, A. thaliana, B. juncea, Ch. reinhardtii, D. bardawil, D.salina, M. sativa, Morus spp.,O. sativa, Salicornia sp., S. tuberosum, winter triticale, Z. mays
AS06 170 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.maritima, B.juncea, D.sanguinalis, E.crus-galli, E.tef, I.pumila, I. walleriana, L.formosana, N.cataria, M. sativa, N.rtanjensis, O.sativa, P.Sogo Yukidian cultivar V3, P.vulgaris, P.sativum, Populus sp., S.superba, S.lycopersicum, S.tuberosum, Z.mays
AS09 603 | Clonality: Polyclonal | Host: Goat | Reactivity: Hen IgY (H&L)