Anti-CRY1 | Cryptochrome 1

Name: Anti-CRY1 | Cryptochrome 1
SKU: AS16 3933
Price: 319 EUR
Availability: InStock
Rating: 5 (1 reviews)

Product no: AS16 3933

AS16 3933 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Data sheet
Product Info

Immunogen:	KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CRY1, UniProt:Q43125, TAIR:AT4G08920
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 1000 (WB)
Expected \| apparent MW:	76.6 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity:	Cajanus cajan, Capsella orientalis, Corchorus capsularis, Fragaria ananassa, Fragaria vesca, Gossypium hirsutum, Medicago truncatula, Nelumbo nucifera, Populus tremula, Zostera marina Species of your interest not listed? Contact us
Not reactive in:	Diatoms

Application Examples

Application example

western blot using anti-CRY1 antibodies

15 µg of total protein from Arabidopsis thaliana etiolated/greening seedling extracted with exact buffer (0.125 M Tris, 1% SDS, 10% Glycerol, 0.05 M Sodium metabisulfite) and denatured at 70°C for 5 min. Samples and Prestained Protein were separated on 10% SDS-PAGE(1.5 mm, 10-well) and blotted 50 min to PVDF using semi-dry. Blots were blocked with 5% nonfat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:5000 in 1% nonfat dry milk for 4h at RT with agitation. The blot was washed as above and developed for 2 min with ECL reagent. Exposure time was 10 min.

Courtesy of Duorong Xu, LMU, Munich, Germany

Background

Background:

CRY1 (Cryptochrome 1) is a flavin-type blue-light photoreceptor with ATP binding and autophosphorylation activity and functions in perception of blue / green ratio of light. It regulates other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception.
Alternative names: Blue light photoreceptor1, Protein BLUE LIGHT UNINHIBITED 1, Protein ELONGATED HYPOCOTYL 4, Protein OUT OF PHASE 2, OOP2.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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Number of reviews: (1)

Andreas | 2021-07-22

0 | 0

The antibody worked well on denatured Arabidopsis whole cell protein extracts at a dilution of 1:2000 (overnight 4�C in 1% milk TBS).