Anti-CRD1 | Cyanobacterial homolog of plant CHL27 cyclase

BCM1 is a positive regulator of Chl biosynthesis in Arabidopsis.a Representative images of 28-day-old WT, bcm1 and BCM1-OX seedlings grown under short-day normal light (120??mol photon?m?2?s?1) conditions. Scale bar, 1?cm. b Scheme of TBS in higher plants. c–g Relative ALA synthesis rate (c), Proto (d), Mg-porphyrin (e), Chl (f), and heme (g) levels in 18-day-old WT, bcm1, and BCM1-OX seedlings grown under the same conditions as in a. Error bars represent SD of eight, three, four, twelve, and six biological replicates, respectively. Letters above histograms indicate significant differences as determined by Tukey’s HSD method (P?<?0.05). h Steady-state levels of the indicated proteins in seedlings analyzed in a were determined by immunoblot analysis using the indicated antibodies. i Steady-state levels of the indicated proteins in 28-day-old WT, gun5-1, cch, gun4-1, gun4-3, chl27, and ch1-2 seedlings, grown under short-day low light (70??mol photons?m?2?s?1) conditions were quantified by immunoblotting using the indicated antibodies. A representative image of the Arabidopsis seedlings analyzed in i is shown. In h, i, Ponceau S-stained membrane strips bearing RbcL were used as loading controls. Numbers below immunoblots represent normalized protein abundances relative to WT seedlings.