Anti-Conglutin gamma | lupin-specific globulin
AS08 335 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Lupinus luteus
- Product Info
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Immunogen: Native protein isolated from lupin seeds
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 33 | 18 kDa in a presence of DTT
- Reactivity
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Confirmed reactivity: Lupinus albus, Lupinus luteus Predicted reactivity: Lupinus albus, Lupinus angustifolius Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Background
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Background: Conglutin gamma, an oligomeric protein, is one of storage proteins of lupin seeds, called also lupin-specific globulin.
- Product Citations
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Selected references: Escudero-Feliu et al. (2023). Functional Association between Storage Protein Mobilization and Redox Signaling in Narrow-Leafed Lupin ( Lupinus angustifolius L.) Seed Germination and Seedling Development. Genes (Basel). 2023 Sep 28;14(10):1889. doi: 10.3390/genes14101889.
Hashemi et al. (2023). Effect of heat treatment on lupin reactivity against rabbit polyclonal antibodies after in vitro gastrointestinal digestion. AOCS, doi.org/10.1002/sfp2.1024.
Villa et al. (2020). Immunoreactivity of Lupine and Soybean Allergens in Foods as Aected by Thermal Processing. Foods. 2020 Feb 27;9(3). pii: E254. doi: 10.3390/foods9030254.
Tomczak et al. (2019). Differences in the immunoreactivity of milk from local farms and from points of purchase. Eur Food Res Technol, Nov 2019.
Foley et al. (2015). Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biol. 2015 Apr 19;15:106. doi: 10.1186/s12870-015-0485-6.
Czubiński et al. (2015). Digestion susceptibility of seed globulins isolated from different lupin species. European Food Research and Technology pp 1-13. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Accessories
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