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Chicken anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse IgG
AS10 839 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)
- Product Info
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Immunogen: Purified Rabbit IgG
Host: Chicken Clonality: Polyclonal Purity: Immunogen affinity purified chicken IgY. Format: Lyophilized Quantity: 0.5 mg Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Rabbit IgG (H&L) Predicted reactivity: Rabbit IgG (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10% (w/v) BSA, Protease/IgG free
0.1% (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Additional information (application): This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.
This antibody was absorbed against solid phase human and mouse serum and IgG.
- Background
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Background: Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
- Product Citations
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Selected references: Vergara-Cruces (2024). Structure of the plant plastid-encoded RNA polymerase. Cell . 2024 Feb 29;187(5):1145-1159.e21. doi: 10.1016/j.cell.2024.01.036. - Protocols
- Antibody protocols
- Reviews:
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Accessories
AS10 1267 | Clonality: Polyclonal | Host:Chicken | Reactivity: Rabbit IgG (H&L)
AS10 829 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (heavy and light chains - H&L)
AS10 831 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)
AS12 2072 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)