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Chicken anti-Rabbit IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse IgG
AS10 835 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)
- Product Info
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Immunogen: Purified Rabbit IgG
Host: Chicken Clonality: Polyclonal Purity: Immunogen affinity purified chicken IgY. Format: Liquid Quantity: 0.5 mg Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) - Reactivity
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Confirmed reactivity: Rabbit IgG (H&L) Predicted reactivity: Rabbit IgG (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA. Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative Additional information (application): This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin rabbit serum proteins or to human or mouse serum or IgG based on immunoelectrophoresis.
- Background
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Background: Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
- Protocols
- Antibody protocols
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