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Chicken anti-Rabbit IgG (H&L), DyLight® 550 conjugated
AS12 1958 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG
- Product Info
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Immunogen: Purified rabbit IgG, whole molecule
Host: Chicken Clonality: Polyclonal Purity: Immunogen affinity purified chicken IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Recommended dilution: 1 : 20-1 : 2000 for most applications - Additional Information
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Additional information: Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide is added as preservative Additional information (application): Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.
No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
- Background
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Background: Chicken anti-Rabbit IgG (H&L) - Affinity Pure, DyLight®550 Conjugate is a secondary antibody conjugated to DyLight® 550, which binds to all rabbit IgG (H&L) in immunological assays.
DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
- Protocols
- Antibody protocols
- Reviews:
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Agnieszka Zienkiewicz | 2013-05-02I have used this antibody for both, Western-blot and immunofluorescence techniques. It works very well in both cases, since it gives highly specific staining practically without backgruond.
Accessories
AS10 831 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)
AS12 2072 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)
AS10 828 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)
AS10 1063 | Clonality: Polyclonal | Host: Chicken | Reactivity: Rabbit IgG (H&L)