Anti-KatG | catalase peroxidase (HPI), cyanobacterial

Product no: AS08 374

AS08 374  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Synechocycstis sp. PCC 6803, E.coli

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  • Product Info
  • Immunogen:

    Recombinant full length KatG protein from Synechocystis sp. PCC 6803 (accessions P73911 and sll1987) with six His-tag on the terminus.

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 µl
    Reconstitution: For reconstitution add 200 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000, 10 µg protein/lane (WB)
    Expected | apparent MW:

    84.4 | 84.4 kDa for Synechocystis sp. PCC 6803

  • Reactivity
  • Confirmed reactivity: Synechocystis sp. PCC 6803
    Predicted reactivity:

    Synechococcus sp. including PCC 7302 and S.elongatus (PCC 6301, PCC 7942); other bacteria


    Species of your interest not listed? Contact us
  • Application Examples
  • Additional Information
  • Additional information (application):

    This product can be sold containing ProClin if requested

  • Background
  • Background:

    Catalase peroxidase (EC 1.11.1.7) is a bifunctional antioxidant enzyme encoded by the katG-gene. It exhibits both, catalase and peroxidase activity which provides a protection against oxidative stress by neutralising hydrogen peroxide. The enzyme is present in a number of bacterial taxa, including cyanobacteria.

  • Product Citations
  • Selected references: Muehler et al. (2024). Stress response in Escherichia coli following sublethal phenalene-1-one mediated antimicrobial photodynamic therapy: an RNA-Seq study. Photochem Photobiol Sci. 2024 Aug 5.doi: 10.1007/s43630-024-00617-3.
    Hakkila et al. (2018). Group 2 Sigma Factors Are Central Regulators of Oxidative Stress Acclimation in Cyanobacteria. Plant Cell Physiol. 2018 Nov 8. doi: 10.1093/pcp/pcy221.
    Wenk et al. (2012). A universally conserved GTPase regulates the oxidative stress response in Escherichia coli. J Bio. Chem. Nov 8.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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Accessories

AS09 501 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.madagascariensis, B.oleracea, Cirtus sp., Fragaria x ananassa, H.vulgare, L. sativus, L. albus, M. perniciosa, N.bentamina, N.tabacum, O.sativa, P. kurroa, P.sativum, P. zeylandica, P.tomentosa, S. italica, S.lycopersicum, S.oleracea, T.aestivum, Z.mays, V. vinifera

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