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Anti-CAH6 | Carbonic anhydrase 6
Product no: AS19 4322
AS19 4322 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamdydomonas reinhardtii
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319
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- Product Info
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Immunogen: Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9 Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 28 kDa - Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Gonium pectorale, Volvox carteri
Species of your interest not listed? Contact usNot reactive in: Lycopersicum esculentum, Neopyropia yezoensis (macroalga), Zea mays - Additional Information
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Additional information (application): Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017.
Extraction method – harvest the cells and add SDS sample buffer. - Background
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Background: CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004).
However, lately localization to flagella has been confirmed Mackinder et al. 2017. - Product Citations
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Selected references: Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044.
Mitra et al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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