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Anti-Beta-tubulin, (loading control antibody), mouse monoclonal
Product no: AS20 4382
AS20 4382 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human
279
€
Delivery:
3-6 business days
- Product Info
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Immunogen: Synthetic peptide derived from the N-terminal of the human beta-tubulin Sub class: IgG2a Host: Mouse Clonality: Monoclonal Purity: IgG2a purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide. Format: Lyophilized Quantity: 100 µg Storage: Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks Tested applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion Expected | apparent MW: 50 kDa - Reactivity
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Confirmed reactivity: Chicken, human, monkey, mouse, rat, rabbit Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
Varied amounts of mouse brain tissue lysate: 10 µg/lane (1), 5 µg/lane (2), 2.5/lane µg (3), 1.25 µg/lane (4) were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 4000 (0.25 µg/ml). Reaction was developed by chemiluminescence.
- Additional Information
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Additional information: 10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide Additional information (application): This antibody recognizes both the native and denatured forms of Beta-tubulin - Background
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Background: Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot. - Reviews:
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