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Anti-Beta-Galactosidase (mouse monoclonal) (clone BG-02)
- Product Info
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Immunogen: beta-Galactosidase purified from E. coli. UniProt: P00722 Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: Immunoglobulin purified on Protein A. Format: Liquid at 1 mg/ml. Quantity: 0.1 mg Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunocyto chemistry (ICC), Western blot (WB) Recommended dilution: 1 µg/ml (WB) Expected | apparent MW: 116.5 kDa - Reactivity
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Confirmed reactivity: Escherichia coli, GalB-tagged fusion proteins - Application Examples
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Purified beta galactosidase from E.coli, non-reduced sample was separated on SDS-PAGE and transferred to a membrane. Protein visualization was done using anti- beta galactosidase monoclonal antibodies at 1 µg/ml, followed by secondary anti-mouse IgG antibodies, HRP conjugated. Reaction was visualized using chemiluminescence, according to manufacture's recommendation.
- Additional Information
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Additional information: This antibody recognizes a non-catalytic centre epitope of beta-galactosidase from E. coli. - Background
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Background: Beta-Galactosidase is an enzyme (EC:3.2.1.23) involved in hydrolysis of terminal non-reducing beta-D-galactose residues into beta-D-galactosides. The protein is encoded by lacZ gene.
Alternative names: Beta-gal, Lactase, GalB - Protocols
- Antibody protocols
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Accessories
AS09 627 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
This product can be purchased in 3 different volumes:
AS16 ECL-N-10, 10 ml. Trial size limited to one per customer
AS16 ECL-N-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
AS20 4515 | Clonality: Monoclonal | Host: Mouse | Reactivity: Beta Galactosidase (E.coli)