Anti-ATUAP1 | UDP-N-acetylglucosamine diphosphorylase 2

 A 1-year-old greehouse grown plant was dissectedinto different tissues, which were then used for enzyme assays and immunoblot analyses. Equal amounts of total protein (7.5 μg) were loaded on each lane. SDS-PAGE was run on a 7.5% gel. Immunoblot was done using Amersham PVDF transfer membrane. Primary antibodies against barley UGPase were used in 1: 1000 dilution. Secondary antibodies (Amersham ECL Rabbit IgG, HRP-Linked Whole Antibody from donkey) were used at 1:10 000.

ff - female flower, mf - male flower, yl - young leaf, ml - mature leaf, sbk - stem bark, sph - stem phloem and cambium, sxy - stem xylem, rxy - root xylem