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Anti-MTP1 | vacuolar Zn2+/H+ antiporter
AS09 485 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum
Replaced by AS23 4900
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 UniProt:Q9ZT63, TAIR:At2g46800
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 100 µl Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 8000 (ELISA), 1 : 1000 (WB) Expected | apparent MW: 43.8 | 43 kDa (Arabidopsis thaliana)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens
Species of your interest not listed? Contact usNot reactive in: Hordeum vulgare, Solanaceae sp. - Application Examples
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Application example
Sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2) separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP1 antibodies (AS09 485, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
- Additional Information
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Additional information: 0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.
MTP1 protein is of low abundance in plant tissues.
Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel - Background
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Background: MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess.
Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein 1 - Product Citations
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Selected references: Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.
Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383.
Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters CollectionMethod for isolation of plant vacuolar membranes
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Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS07 213 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants including A.comosus, A.thaliana, C.sativus, C. australis R.Br, C.reinhardtii, F. margarita Swingle, H.vulgare, L.esculentum, L.longiflorum, Malus x domestica Borkh. c.v. Fuji, M. truncatula, M.crystallinum, N.tabacum, N.caerulescens, O.sativa, P.hybrida cv. Mitchell, Populus sp., P.vittata, Thellungiella sp., T. aestivum, Z.mays, V. vinifera | Cellular [compartment marker] of tonoplast membrane
AS07 260 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane
Benefits of using this antibody