Anti-ATG5 | Autophagy-related protein 5

Name: Anti-ATG5 | Autophagy-related protein 5
SKU: AS15 3060
Price: 237 EUR
Availability: InStock

Product no: AS15 3060

AS15 3060 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

237 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 �l
Reconstitution:	For reconstitution add 50 �l of sterile water
Storage:	Store lyophilized/reconstituted at -20�C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 1000 (WB)

Reactivity
Confirmed reactivity: Recombinant ATG5 of Arabidopsis thaliana

Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information

Additional information:

This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed. It does not react with 6xHis-ATG7.

This product can be sold containing ProClin if requested.

Background

Background:

ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling. It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes.

Product Citations

Selected references:

Young et al. (2019). A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes. Autophagy. 2019 Jun;15(6):941-959. doi: 10.1080/15548627.2019.1569915.

Protocols
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