Anti-FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)

Product no: AS05 094A

AS05 094A  |  Clonality: Polyclonal  | Host: Rabbit  |  Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated  peptide chosen from AtFtsH6 protein sequence of Arabidopsis thaliana  Q1PDW5

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: For reconstitution add 100 µl of sterile water/tube
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1 500-1 : 1000 (WB)
    Expected | apparent MW: 74.36 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity:

    Arabidopsis thaliana

    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application examples:

    Reactant: Arabidopsis thaliana (Thale cress)

    Application: Western Blotting

    Pudmed ID: 27561243

    Journal: Nat Commun

    Figure Number: 6B

    Published Date: 2016-08-26

    First Author: Sedaghatmehr, M., Mueller-Roeber, B., et al.

    Impact Factor: 13.783

    Open Publication

    Metalloprotease FtsH6 is involved in thermopriming.(a) FtsH6 expression in Arabidopsis N13 and Col-0 seedlings during the memory phase compared to unprimed controls. FC, fold change. Error bars indicate means±s.d. of three independent biological replicates each containing a pool of ?160 seedlings. (b) Immunoblot analysis of FtsH6 protein in accessions Col-0 and N13 at different time points of the memory phase. Immunodetection was performed using anti-FtsH6 antibody (top panels). RbcL, Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panels). Note the absence of FtsH6 protein in Col-0 unprimed samples and in N13 unprimed and primed samples. (c) Immunoblot analysis of HSP21 protein in ftsh6 mutant (Salk_012429) and Col-0 seedlings after the first step of the priming treatment (90?min, 37?°C) and during the thermomemory phase. Immunodetection was performed using anti-HSP21 antibody (top panel). RbcL, ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panel). (d) Signals of the immunoblot analyses were quantified using ImageJ and normalized to the amount of RbcL in the same samples. Means±s.d. are given (n=3, independent biological replicates each representing a pool of ?100 seedlings grown on five plates; gel blots used for the quantification are shown in Supplementary Fig. 10). Asterisks indicate statistically significant difference (P<0.05; Student's t-test) from the Col-0 control. kDa, kilo Dalton.


    Reactant: Arabidopsis thaliana (Thale cress)

    Application: Western Blotting

    Pudmed ID: 27561243

    Journal: Nat Commun

    Figure Number: 6C

    Published Date: 2016-08-26

    First Author: Sedaghatmehr, M., Mueller-Roeber, B., et al.

    Impact Factor: 13.783

    Open Publication

    Metalloprotease FtsH6 is involved in thermopriming.(a) FtsH6 expression in Arabidopsis N13 and Col-0 seedlings during the memory phase compared to unprimed controls. FC, fold change. Error bars indicate means±s.d. of three independent biological replicates each containing a pool of ?160 seedlings. (b) Immunoblot analysis of FtsH6 protein in accessions Col-0 and N13 at different time points of the memory phase. Immunodetection was performed using anti-FtsH6 antibody (top panels). RbcL, Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panels). Note the absence of FtsH6 protein in Col-0 unprimed samples and in N13 unprimed and primed samples. (c) Immunoblot analysis of HSP21 protein in ftsh6 mutant (Salk_012429) and Col-0 seedlings after the first step of the priming treatment (90?min, 37?°C) and during the thermomemory phase. Immunodetection was performed using anti-HSP21 antibody (top panel). RbcL, ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panel). (d) Signals of the immunoblot analyses were quantified using ImageJ and normalized to the amount of RbcL in the same samples. Means±s.d. are given (n=3, independent biological replicates each representing a pool of ?100 seedlings grown on five plates; gel blots used for the quantification are shown in Supplementary Fig. 10). Asterisks indicate statistically significant difference (P<0.05; Student's t-test) from the Col-0 control. kDa, kilo Dalton.

  • Background
  • Background:

    Degradation of the most abundant membrane protein on earth, the light-harvesting complex of Photosystem II (LHC II), is highly regulated under various environmental conditions, e.g., light stress, to prevent photochemical damage to the reaction center. FtsH6 is proposed to be a general LHC II protease and FtsH6-dependent LHC II proteolysis can be  a feature of all higher plants.

  • Product Citations
  • Selected references: Sedaghatmehr et al. (2022) Heat shock factor HSFA2 fine-tunes resetting of thermomemory via plastidic metalloprotease FtsH6. J Exp Bot. 2022 Jun 15:erac257. doi: 10.1093/jxb/erac257. Epub ahead of print. PMID: 35705109.
    Sedaghatmehr et al. (2016). The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis. Nat Commun. 2016 Aug 26;7:12439. doi: 10.1038/ncomms12439.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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