Anti-ANN-1 | Annexin-1

Product no: AS18 4166

AS18 4166   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

228
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana Annexin-1, UniProt Q9SYT0, TAIR AT1G35720
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 36 kDa (before processing)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Western blot using anti-Annexin 1 antibodies

    Recombinant Ann1 (1), Ann4 OE (2). Leaves of Arabidopsis thaliana were ground in liquid nitrogen and total protein was extracted using extraction buffer: Tris pH-8.0 (1M) 5%, EDTA pH-8.0 (0.5 M) 0.2%, β-Mercaptoethanol 0.05%, PMSF (1%) 0.05%, Triton X-100 1%, in distilled water. For each sample, 50 µg of extracted protein was taken, diluted with distilled water and added with 4xLaemli Sample buffer. The samples were denatured at 95℃ for 5 min and were separated on 12 % SDS-PAGE. The samples were blotted for 2 h to PVDF membrane using tank/wet transfer at 4℃ with constant stirring. The blots were blocked with blocking solution (5% dried milk in 1xTBS) for overnight at 4℃ with agitation. Blots were incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation in 5% dried milk in 1xTBS. The antibody solution was decanted and the blots were rinsed briefly, then washed once for 15 min and 3 times for 5 min in 1xTBS at RT with agitation. Blots were incubated in secondary antibody (Goat anti-rabbit IgG (HRP conjugated)) diluted to 1:50 000 in 5% dried milk in 1xTBS for 1h at RT with agitation. The blots were washed as mentioned above and developed for 5 min with chemiluminescent detection reagent in dark room. The exposure time was 10 min.

    Courtesy Dr. dr Umesh Tanwar, Departament of Plant Biochemistry Institute of Biochemistry and Biophysics PAS, Poland
  • Additional Information
  • Additional information (application): Antibody is detecting recombinant Annexin-1 of Arabidopsis thaliana. Its reactivity on endogenous form remains to be confirmed.
  • Background
  • Background: ANN-1 | Annexin-1 displays peroxidase activity and may counteract oxidative stress.

    Alternative names: ANNAT1, ANX23-ATH, ATOXY5, OXY5, Annexin D1, AnnAt1.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info
AS19 4294  |  Clonality: Polyclonal  |  Host: Rabbit/Hen
1553 €

AS06 125  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. maritima, A. thaliana, B. juncea, Ch. reinhardtii, D. bardawil, D.salina, M. sativa, Morus spp.,O. sativa, Salicornia sp., S. tuberosum, winter triticale, Z. mays

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
Close