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Anti-AOX1 | Algal Alternative oxidase 1
AS06 152 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of mitochondrial inner membrane
- Product Info
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Immunogen: whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 10 000 (WB) Expected | apparent MW: 36 | 36 kDa (Chlamydomonas reainhardii)
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Nannochloropsis gaditana, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis
Species of your interest not listed? Contact usNot reactive in: Cyanidioschyzon merolae - Additional Information
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Additional information: Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane - Background
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Background: The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Alternative oxidase expression is influenced by different stress stimuli.
- Product Citations
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Selected references: Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9
Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtii
Perlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577.
Kaye et al. (2019). The mitochondrial alternative oxidase from Chlamydomonas reinhardtii enables survival in high light.J Biol Chem. 2019 Jan 25;294(4):1380-1395. doi: 10.1074/jbc.RA118.004667.
Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
Inman (2013). Characterization of the Alternative Oxidase from the Psychrophilic Green Alga Chlamydomonas sp. UWO241. Thesis.
Bohne et al. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLOS, Open Access. - Protocols
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Isolation of mitochondrial fraction
Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Marie Bertrand | 2023-09-20Used on chlamydomonas. Good signal.Andrew Castonguay | 2020-08-08Loaded 20 ug of C. reinhardtii whole cell extract from freeze thaw method. Blocked with TBS+0.1%Tween, 5% milk for 1 hr at rm temp or overnight at 4 deg C. Antibody diluted 1:10,000 in TBS+0.1%Tween, 2% milk. Primary incubation overnight with gentle agitation at 4 deg C. 5 washes in between primary and secondary, volume for all antibody incubations and washes was 25 ml, washes at room temp. Immunoblot with HRP-conjugated secondary (1:20,000, 0.1%TBST) was clean with these conditions, however, longer exposure will yield a few additional bands, one at ~60 kDa and another at ~30 kDa (12% polyacrylamide).
Accessories
AS06 151 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of mitochondrial inner membrane
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)