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Anti-FOX1 | plasma membrane ferroxidase FLP1
AS06 120 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
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Immunogen: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: immunofluorescence (IF), Western blot (WB) Recommended dilution: 1 : 1000-1 : 5000 (WB) Expected | apparent MW: 122 | 120 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Chlamydomonas reinhardtii Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.
Additional information (application): This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.
- Background
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Background: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.
- Product Citations
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Selected references: Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell 1(5):736-757
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Marita Hermann | 2012-08-16The FOX1 antibody was tested on whole cell extracts from Chlamydomonas reinhardtii grown both under iron depleted and iron replete conditions. 30 microgram total proteins (quantified by BCA assay) were loaded in each well, separeted on a 13% SDS-PAGE and transferred onto a nitrocellulose membrane. Blocking was performed in PBS 2% milk at room temperature for 1 hour incubation with primary Agrisera FOX1 was also performed in PBS 2% milk by using the suggested 1:1000 dilution. After three washed with PBS 2% milk, secondary anti-rabbit (in PBS 2% milk) was incubated for 1.5 h at a dilution of 1:10,000. Detection was perfomed with a standard ECL assay. Bands of the correct molecular weight could be seen on the film, but background was pretty high and also several aspecific bands were present.
Accessories
AS07 260 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane
Benefits of using this antibody
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)