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Anti-AKT1 | Potassium channel AKT1
Product no: AS09 516
AS09 516 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
448
€
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 200 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 50 with 125I (WB) Expected | apparent MW: 96.9 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1 Additional information (application): In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell) - Background
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Background: AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
- Product Citations
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Selected references: Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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