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Anti-AGO3-PAZ | Argonaute 3 PAZ domain (Chlamydomonas)
Product no: AS14 2799
AS14 2799 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii
329
€
Customer reviews
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated peptide derived from C-terminal of AGO3 protein of Chlamydomonas reinhardtii
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipiatation (IP), Western blot (WB) Recommended dilution: 1: 2500 (WB) Expected | apparent MW: 116.4 | 130 kDa - Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii
- Application Examples
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Application example 
Total proteins of Chlamydomonas reinhardtii saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-AGO3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (anti-rabbit, DyLight® 800 conjugated, AS12 2460 Agrisera) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.
Courtesy Dr. Betty Chung, Department of Plant Sciences, University of Cambridge, UK - Background
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Background: Argonaute 3(AGO3) of Chlamydmonas reinhardii is cytoplasmic enriched and associated with polysomes and miRNAs (Chung et al. 2019).
- Product Citations
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Selected references: Chung et al. (2019) Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression. Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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