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Anti-ACT 2,8,11 | Actin 2, 8, 11 (clone mAb13a)
Product no: AS16 3140
AS16 3140 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Nicotiana benthamiana
387
€
Delivery:
7-10 business days
- Product Info
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Immunogen: Full-length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E.coli, Uniprot: Q96292
Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: IgG1 immunoglobulin purified on Protein G in 0.1 M Sodium Phosphate, pH 7.4, 0.15 M NaCl, 0.05% (w/v) sodium azide. Format: Liquid Quantity: 100 µg Storage: Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1-2 µg/ml (WB) Expected | apparent MW: 45 | 45 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum!!AIR8!!Arabidopsis thaliana, Nicotiana tabacum Predicted reactivity: Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp., Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp. japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujuba
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: This antibody is a total IgG fraction, purified on a protein G column. It recognizes actin isoforms ACT2, 8, and 11.
Additional information (application): Exact working dilution needs to be determined by end user - Background
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Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. - Product Citations
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Selected references: Kandasamy, M.K., et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell May;24(5):2041-57. doi: 10.1105/tpc.111.095281.
Kandasamy, M.K., et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54.
Kandasamy, M.K., et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-9. - Reviews:
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Accessories
AS16 3139 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
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