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Anti-ACP3 | Activated Caspase 3 (p20/p17 subunit)
AS21 4569 Host: Rabbit | Reactivity: Human, mouse, rat
- Product Info
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Immunogen: KLH-conjugated synthetic peptide corresponding to the human caspase 3 cleavage site, 6 aa (CGIETD) UniProt: P42574 Host: Rabbit Purity: Serum. Contains 0.05% sodium azide. Format: Liquid Quantity: 100 µl Storage: Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunolocalisation (IL), Western blot (WB) Recommended dilution: 1: 500 - 1: 1000 (IL), 1:3000-1:1000 (WB)
Expected | apparent MW: 31.6 | 17 and 19 kDa - Reactivity
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Confirmed reactivity: Human, Mouse and Rat Predicted reactivity: Species of your interest not listed? Contact us - Application Examples
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Caspase 3 activation in neurons accumulating wild-type APP (ref.3). NT2 neurons (neurally differentiated human NT2 embryonic carcinoma cells) were infected with adenovirus vector expressing β-galactosidase (upper panel) or APP (lower panel), fixed 48 h later, and triply stained for the N-terminus of APP (with antibody P2-1) or β-gal (with antibody against β-gal), chromosomal DNA (Hoechst), and activated caspase 3 subunits (with antibody ACP3). Some neurons accumulating APP are strongly immunostained with ACP3 (arrows), whereas neurons accumulating β-gal are hardly labeled (arrowheads). β-gal APP Hoechst ACP3 β-gal or APP. Sercondary antibody was goat anti-rabbit IgG, FITC conjugated.
- Additional Information
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Additional information: ACP3 | Activated Caspase 3 (p20/p17 subunit) Additional information (application): The antibody does not react with the proenzyme p32 - Background
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Background: Caspases are a family of cysteine proteases which play essential roles in apoptosis. Among them, Caspase 3 is a frequently activated death protease, catalyzing the specific cleavage of many key cellular proteins. Caspase 3 is synthesized as an inactive 32 kDa pro-enzyme which undergo proteolytic processing in response to apoptotic stimulation to produce the active form which consists of the p20/p17, and p12 subunits. Caspase 3 is the predominant caspase involved in the cleavage of Alzheimer amyloid precursor protein (APP), which is associated with neuronal death in Alzheimer ‘s disease. An antibody (named ACP3) against activated caspase 3 was raised in rabbit. This antibody recognizes the active form of human caspase 3, p20/p17 subunit but does not recognize the proenzyme p32. - Product Citations
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Selected references: Nishimura et al (2003). Upregulation and antiapoptotic role of endogenous Alzheimer amyloid precursor protein in dorsal root ganglion neurons. Exp Cell Res. 2003 Jun 10;286(2):241-51. doi: 10.1016/s0014-4827(03)00066-1. PMID: 12749853.
Nishimura et al. (2002) Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ. 2002 Feb;9(2):199-208. doi: 10.1038/sj.cdd.4400931. PMID: 11840170. - Protocols
- Antibody protocols
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)