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Anti-His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)
Product no: AS15 2935
AS15 2935 | Clonality: monoclonal | Host: mouse | Reactivity: Polyhistidine tagged proteins (N- or C-terminus label)
455
€
Delivery:
3-6 business days
- Product Info
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Immunogen: Polyhistidine (HHHHHH) tagged polypeptide
Host: Mouse Clonality: Monoclonal Purity: Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide. Format: Lyophilized Quantity: 100 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C. Tested applications: Western blot (WB) Recommended dilution: 1: 5000 - 10 000 (WB) Expected | apparent MW: depends upon fusion partner - Reactivity
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Confirmed reactivity: 6xHis tagged proteins - Application Examples
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Indicated amount of 6xHis tagged, 60 kDa protein has been loaded to each lane. Primary antibody incubation (AS15 2935): 1: 10 000 1h/RT Followed by three washes and reaction was developed using alkaline phosphatase substrate.
- Additional Information
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Additional information: Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4)
Concentration: 0.1mg/ml
Additional information (application): Final primary antibody dilution, depend upon amount of Hisx6 tagged protein in analyzed sample. - Background
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Background: Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag. - Reviews:
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