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Anti-FNR2 | Ferredoxin NADP Reductase, isoprotein 2 (leaf)
Product no: AS20 4438
AS20 4438 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays
341
€
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- Product Info
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Immunogen: Purified full length, tag cleaved, recombinant maize leaf FNR2, UniProt: Q9SLP5, sharing homology with Arabidopsis thaliana FNR2, UniProt: Q8W493 Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized. Format: Liquid at 1 mg/ml. Quantity: 100 µg Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1: 2000 - 1: 50 000 (WB) Expected | apparent MW: 39.3 kDa | 35.57 kDa (FNR2, Zea mays) - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Zea mays Predicted reactivity: Dichanthelium oligosanthes, Glycine max, Sorghum bicolor
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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10 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Molecular weight of mature forms of maize L-FNRs:
34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)
Cellular distribution of maize FNR isoforms
BSC - bundle sheah cells, protein load 4 µg/well
MC- mesophyll cells, protein load 4 µg/well
Primary antibody: 1: 50 000
Anti-FNR2 Antibody cross reacts with other leaf maize FNR isoforms, FNR1 and FNR3. - Additional Information
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Additional information (application): This antibody is also detecting other maize L-FNRs, FNR1, FNR3 (reference image below) and Arabidopsis thaliana FNR1 (leaf). - Background
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Background: FNR2 | Ferredoxin NADP Reductase, isoprotein 2 (leaf) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Alternative names: FNR - Product Citations
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Selected references: Twachtman et al. (2012). N-terminal Structure of Maize ferredoxin:NADP+ Reductase Determines Recruitment Into Different Thylakoid Membrane Complexes. Plant Cell. 24(7):2979-91. doi: 10.1105/tpc.111.094532.
Twachtmann et al. (2012). N-terminal Structure of Maize ferredoxin:NADP+ Reductase Determines Recruitment Into Different Thylakoid Membrane Complexes. Plant Cell. 2012 Jul;24(7):2979-91. doi: 10.1105/tpc.111.094532. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
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