Anti-ATG5 | Autophagy protein 5

Name: Anti-ATG5 | Autophagy protein 5
SKU: AS19 4276
Price: 319 EUR
Availability: InStock

Product no: AS19 4276

AS19 4276 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Info

Immunogen:	Full-length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl, of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	38.5 \| 50 kDa (in wilde type plants) and ca. 38 kDa in autophagy defective mutants

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Solanum lycopersicum, Solanum tuberosum
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Additional Information
Additional information (application): In wild-type plants almost all ATG5 is conjugated to ATG12.

This item can be sold containing ProClin
Background

Background:

ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes.

Alternative names: Protein autophagy 5, AtAPG5.

Product Citations
Selected references: Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.
Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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