Anti-RAD23c | Ubiquitin receptor RAD23c

Name: Anti-RAD23c | Ubiquitin receptor RAD23c
SKU: AS19 4271
Price: 319 EUR
Availability: InStock

Product no: AS19 4271

AS19 4271 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Info

Immunogen:	Full-length, His-tagged recombinant RAD23c of Arabidopsis thaliana protein sequence, UniProt:Q84L31-1, TAIR: At3g02540, overexpressed in E.coli
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl, of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	44.2 \| ca. 61 kDa

Reactivity
Confirmed reactivity: Arabidopsis thaliana

Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information

Additional information (application):

Antibodies recognize recombinant RAD23b and RAD23c proteins equally well but do not recognize DSK2a/b orDDI1 based on immunoblot analyses of corresponding mutants. Farmer et al. (2010).

Background

Background:

RAD23c (Ubiquitin receptor RAD23c) binds and presumably selects ubiquitin-conjugates for destruction. Prefers multiubiquitin chains rather than single ubiquitins, with a binding affinity for 'Lys-48'-linked ubiquitin chains. Acts as a ubiquitin receptor that associates with the 26S proteasomal docking subunit RPN10 for the indirect recognition of ubiquitinated substrates of ubiquitin/26S proteasome-mediated proteolysis (UPP). RAD23 proteins play an essential role in the cell cycle, morphology, and fertility of plants through their delivery of UPS (ubiquitin/26S proteasome system) substrates to the 26S proteasome.

Alternative names: AtRAD23c, Putative DNA repair protein RAD23-3, RAD23-like protein 3, AtRAD23-3.

Product Citations

Selected references:

Farmer et al. (2010). The RAD23 family provides an essential connection between the 26S proteasome and ubiquitylated proteins in Arabidopsis. Plant Cell. 2010 Jan;22(1):124-42. doi: 10.1105/tpc.109.072660.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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