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Anti-Heteromannan (monoclonal, clone LM21)
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
- Product Info
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Sub class: IgM Immunogen: Polysaccharide Heteromannan Host: Rat Clonality: Monoclonal Purity: Cell culture supernatant. Format: Liquid Quantity: 1 ml Storage: Store at +4°C (short term) and at -20°C (long term). Tested applications: ELISA (ELISA), Immuniflourescence (IF) Recommended dilution: 1:10 (ELISA, IF) - Reactivity
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Confirmed reactivity: Higher plants, ferns and mosses Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Type of material: Fresh Arabidopsis thaliana stem cut on vibratome after fixation
Fixation: paraformaldehyde (4%)
Hydrophilization: no
Cell wall digestion: no
Membrane permeabilization: no
Antigen retrieval: no
Blocking buffer: 5% milk in PBS
Washing buffer: 5% milk in PBS-T
Primary antibody dilution and incubation time: 1:20 incubation 90 minutes at room temperature
Secondary antibody dilution and incubation time and supplier: Thermo Fisher Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 633 (A-21094)
Co-staining of the nucleus (DAPI): no
Cell wall and nucleus staining: noneCourtesy of Dr. Jan Łyczakowski, Jagiellonian University, Kraków, Poland
- Additional Information
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Additional information: Contains 0.05% Sodium Azide.
No cross-reactivity with other polymers
This antibody recognises B-linked mannan polysaccharides of plant cell walls.
LM21 binds effectively to B-(1-4)-manno-oligosaccharides from DP2 to DP5. - Background
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Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. - Product Citations
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Selected references: Marcus et al. (2010). Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct;64(2):191-203.doi:0.1111/j.1365-313X.2010.04319.x. - Protocols
- Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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Accessories
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
This set contains 3 Cell wall antibodies of your choice, chosen from a drop down menu.
AS16 3116-1ml | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Cyamopsis tetragonoloba
From the laboratory of Michael G. Hahn, PhD, University of Georgia