Anti-FtsZ | Procaryotic cell division GTPase

Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., <e
Species of your interest not listed? Contact usm>Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038, Xanthomonas oryzae

Alysiella filiformis, B. subtilis, Haloferax mediterranei (Listera sp., Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus (strain MRSA252), cyanobacteria

 5 µg of total protein from Synechocystis sp. (1), E.coli DH5a (2), E. coli (3),  extracted with Agrisera PEB extraction buffer were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Advance blocking reagent  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.

Total protein extract from E. coli CFT073: 8µg (1), 12µg (2), 16µg (3). Proteins were separated on 10% SDS-PAGE and blotted to PVDF. Blocked with 5 % non-fat milk in TBS-T for 1 hour. Blot was incubated in the primary antibody at a dilution of 1 :10 000 for 1 h at RT with agitation. Secondary antibody (anti-rabbit IgG, HRP conjugated, Agrisera, AS09 602) were diluted to 1 : 50 000 and blot was incubated for 1h at RT with agitation. Immunodetection was performed using chemiluminescent detection method for 3 min. Scan was made after 30 sec.

Courtesy of Dr. Marta Kicia, Wroclaw Medical University, Poland

FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.