Tag antibody guide
Antibodies to tags are the most used and published antibodies in research. Where a specific antibody is missing, tag antibodies can be used to facilitate studies on a target protein in transgenic organisms. Agrisera's collection contains over 200 antibodies to variety of used tags, both the most popular ones, and those that are less common.
This guide aims to provide you with more information on different tags, as well as tag antibody recommendations.
This guide aims to provide you with more information on different tags, as well as tag antibody recommendations.
Common tags
Tags can be divided into separate categories, based on their function and inherit properties. Below is a list of different types of tags, as well as short descriptions of these. In the table of tags further down, the abbreviation for the type can be found in the right-most column.
• Affinity Tag (AT): Binds specifically to a partner molecule, allowing one-step purification of the protein using affinity chromatography methods.
• Affinity Tag (AT): Binds specifically to a partner molecule, allowing one-step purification of the protein using affinity chromatography methods.
• EpitopeTag (ET): A short, defined peptide sequence, often 6-15 amino acids long, that has no intrinsic biological function or activity. The primary role of an epitope tag is to provide a means for the detection or purification of the target protein.
• Fluorescent Tag (FT): Enables the detection and localization of proteins through their ability to emit fluorescence when excited by specific wavelengths of light.
• Fuctional/Enzyme Tag (FET): Has a biological activity, such as enzymatic or catalytic properties, often used for performing specific biochemical reactions or assays.
• Reporter Protein Tag (RPT): Provides a measurable signal, allowing monitoring of the expression or activity of a protein in a system.
• Selectable Marker Tag (SMT): Enables selection or differentiation based on resistance to a specific substance.
• Solubility/Expression Enhancer Tag (SEET): Improves the solubility, stability, or expression of the target protein in systems where proteins may otherwise aggregate or be poorly expressed.
• Fluorescent Tag (FT): Enables the detection and localization of proteins through their ability to emit fluorescence when excited by specific wavelengths of light.
• Fuctional/Enzyme Tag (FET): Has a biological activity, such as enzymatic or catalytic properties, often used for performing specific biochemical reactions or assays.
• Reporter Protein Tag (RPT): Provides a measurable signal, allowing monitoring of the expression or activity of a protein in a system.
• Selectable Marker Tag (SMT): Enables selection or differentiation based on resistance to a specific substance.
• Solubility/Expression Enhancer Tag (SEET): Improves the solubility, stability, or expression of the target protein in systems where proteins may otherwise aggregate or be poorly expressed.
| Tag | Sequence/ origin | Agrisera antibodies | Molecular weight | Common use | Type |
|---|---|---|---|---|---|
| aadA1 tag (Aminoglycoside adenyltransferase tag) | Full-length aadA1 enzyme (267 amino acids) | Anti-aadA1 | ~30 kDa | Selectable marker for genetic transformation of plants. | SMT |
| BAR tag (Phosphinothricin N-acetyltransferase tag) | Phosphinothricin N-acetyltransferase | Anti-BAR | 3-5 kDa | Selectable marker for genetic transformation of plants. | SMT |
| BC2 tag (Binding Component 2 tag) | P D R K A A V S H W Q Q | Anti-BC2 | ~1.4 kDa | AT, ET | |
| Biotin tag | Biotin | Anti-Biotin | 244 Da | AT | |
| Ble tag (Bleomycin resistance protein tag) | Bleomycin resistance protein from Streptoalloteichus hindustanus | Anti-Ble | ~1.9 kDa | Selectable marker for genetic transformation of plants. | SMT |
| BirA tag (mutated/TurboID) | Recombinant, mutated BirA protein from Escherichia coli | Anti-BirA | 35 kDa | Studies of protein interactions in different types of cells, organs and developmental stages. Proximity labelling. | AT |
| c-Myc tag | Anti-c-Myc | 1.2 kDa | Protein detection in Western blotting, immunofluorescence, and immunoprecipitation. | ET | |
| CFP tag (Cyan fluorescent protein) | Variant of GFP | Anti-CFP | ~27 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT |
| CBP tag (Calmodulin binding peptide tag) | K R R W K K N F I A V S A A N R F K K I S S S G A L | Anti-CBP | ~3 kDa | Can also act as an epitope tag, | AT |
| CRISPR tag (Cas9) | Streptococcus thermophilus Cas9/Csn1 protein | Anti-Cas9 | ~160 kDa | FET | |
| DYKDDDDK (Binds to Sigma FLAG®) | D Y K D D D D K | Anti-DYKDDDDK | ~1 kDa | Detection and purification; known for its small size and strong antibody affinity. Can also be considered an epitope tag but is often used for purification. | AT, ET |
| FITC tag (Fluorescein isothiocyanate) | Derivative of fluorescein, functionalized with an isothiocyanate group | Anti-FITC | ~0.39 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT |
| GAL4 tag (Regulatory protein GAL4 tag) | Full-length GAL4 protein (881 amino acids) | Anti-GAL4 | 99 kDa | FET | |
| GFP tag (Green fluorescent protein) | Aequorea victoria jellyfish (220 amino acids) | Anti-GFP | ~27 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT |
| GST tag (Glutathione S-transferase tag) | Full-length GST protein (211 amino acids) | Anti-GST | ~26 kDa | Protein purification and pull-down assays. | AT |
| GUS tag (Beta-glucuronidase tag) | Derived from Escherichia coli GUS protein | Anti-GUS | 70-80 kDa | RPT | |
| HA tag (Hemagglutinin tag) | Y P Y D V P D Y A | Anti-HA | 1.1 kDa | Immunoprecipitation and Western blotting. | ET |
| Halo tag (Haloalkane dehalogenase tag) | Full-length HaloTag protein (297 amino acids) | Anti-Halo tag | 33 kDa | AT | |
| His tag (Polyhistidine tag) | Typically HHH or HHHHHH | Anti-His | ~0.4 kDa (3xHis), ~0.8 kDa (6xHis) | Protein purification via immobilized metal affinity chromatography (IMAC). | AT |
| HSV tag (Herpes simplex virus tag) | S Q P E L A P E D P E D | Anti-HSV | ~1.3 kDa | ET | |
| KT3 tag | K P P T P P P E P E T | Anti-KT3 | ~1.2 kDa | ET | |
| LUC tag (Luciferase tag) | Derived from LUC protein sequence of Photinus pyralis | Anti-LUC | 61 kDa | RPT | |
| MBP tag (Maltose binding protein tag) | Full-length MBP protein from Escherichia coli | Anti-MBP | 42.5 kDa | Studies of protein expression using a wide range of applications. Overlap with solubility enhancers, but widely used as an affinity tag. | AT, SEET |
| mCherry tag | DsRed protein from Discosoma sp. coral (236 amino acids) | Anti-mCherry | ~26 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT |
| mOrange tag | DsRed protein from Discosoma sp. coral | ~27 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT | |
| Myc | E Q K L I S E E D L | ||||
| RFP tag (Red fluorescent protein) | DsRed protein from Discosoma sp. coral | Anti-RFP | ~26 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT |
| S tag (Streptavidin tag) | K E T A A A K F E R Q H M D S | Anti-S | 1.75 kDa | ET | |
| Strep-tag II tag | W S H P Q F E K | Anti-Strep | ~1 kDa | High-affinity binding to Strep-Tactin for purification purposes. | AT |
| Sumo tag (Small ubiquitin-like modifier tag) | Often used as a solubility enhancer, but also for affinity purification. | SEET, AT | |||
| tdTomato tag | Tandem dimer of RFP | ~54 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT | |
| T7 tag (Derived from T7 bacteriophage gene 10) | M A S M T G G Q Q M G | Anti-T7 | 1.1 kDa | Detection in Western blotting and immunoprecipitation. | ET |
| TAP tag (Tandem Affinity Purification tag) | C S S G A L D Y D I P T T A S E N L Y F Q | Anti-TAP | 35-40 kDa | Rapid purification of low abundance complexes. TAP approach can be combined with mass spectrometry to allow identification of protein interactions with a given target protein. | AT |
| TRITC tag (Tetramethylrhodamine) | Derivative of rhodamine, functionalized with an isothiocyanate group | Anti-TRITC | ~0.44 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT |
| Trx tag (Thioredoxin 1 Fusion protein tag) | Full-length Trx recombinant protein (104 amino acids) | Anti-Trx | 12 kDa | Fusion tag in a number of molecular biology applications. | SEET |
| V5 tag (Derived from V protein of simian virus 5) | G K P I P N P L L G L D S T | Anti-V5 | ~1.4 kDa | Protein detection and immunoprecipitation. | ET |
| VSV-G tag | Y T D I E M N R L G K | Anti-VSV-G | ~1.3 kDa | Protein detection and purification. | ET |
| YFP tag | Variant of GFP | Anti-YFP | ~26 kDa | Monitoring protein localization, movement, and interactions in live cells, confirming the expression of tagged proteins, studying gene expression and protein-protein interactions. | FT |
Tag antibodies Q&A
Common questions and asnwers related to tags and tag antibodies.
Most tag antibodies are species-independent since they recognize artificial peptide sequences, not endogenous proteins.
Tag antibodies bind specifically to the tagged protein. These antibody-protein complexes can be pulled down using beads, isolating the tagged protein and its interacting partners.
Antibodies can be utilized in live cell imaging, using flow cytometry, to detect membrane surface proteins. In this application, fluorescent tags like GFP are commonly used. Antibody-based detection typically requires cell fixation and permeabilization, making it unsuitable for live cells unless specialized cell-permeable antibodies are used.
| Proximity labelling is a laboratory technique which makes it possible to label proteins (in this case by biotinylation), proximal to protein of interest. This allows the study of protein interactome networks in different types of cells and organs, as well as different developmental stages. The technique is achieved by a gene fusion in a living cell, between the protein of interest and BioID (BirA). The TurboID variant is a biotin ligase, with substantially higher activity, which provides higher temporal resolution and broader application in vivo. The labelling time is just 10 minutes in RT, compared to the over 18 hours of labelling time required for BioID. Agrisera offers a new polyclonal antibody, prepared to a recombinant mutated BirA protein from E.coli, called TurboID. anti-BirA (mutated/TurboID) The proximity labelling technique is described in various publications, e.g.: Zhang et al. (2020). TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks.J Vis Exp. 2020 May 17;(159).doi: 10.3791/60728. Cho et al. (2020). Proximity labeling in mammalian cells with TurboID and split-TurboID.Nat Protoc. 2020 Dec;15(12):3971-3999. doi: 10.1038/s41596-020-0399-0. Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. |
Popular Agrisera tag antibodies
Agrisera offers a vast variety of antibodies to different tags. Below is a list of some of our most popular tag antibodies.
Browse the complete list here.
Browse the complete list here.
AS20 4440 | Clonality: Polyclonal | Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
329 €
AS11 1775 | Clonality: Polyclonal | Host: Rabbit | Reactivity:C-terminal of YFP
329 €
AS16 3690 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cas9 from Streptococcus thermophilus
329 €
AS20 4443 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Green Fluorescence Protein (GFP)
329 €
AS12 2220 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human influenza hemagglutinin (HA) tagged proteins (strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype)
469 €
AS21 4525 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mNeonGreen | Fluorescent Protein
329 €
AS21 4527 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Strep-tag®II-Taged proteins
329 €
Check out the complete collection of Agrisera epitope tag antibodies
Make use of the filter function when looking for your tag antibody of interest! For each tag antibody group, you can filter the list of products by Tested Applications and Clonality, to easier find exactly what you need.
