Agrisera supports protein detection in your laboratory with tailored reagents with the following features:
For chemiluminescent detection of the activity of HRP (Horseradish Peroxidase enzyme), coupled to a secondary antibody, we recommend using: AgriseraECL Bright (AS16 ECL-N), for low pico to mid-femtogram detection (for proteins of medium and high expression), or the enhanced AgriseraECL SuperBright (AS16 ECL-S), for extreme low femtogram detection (for proteins of low expression). Both reagents are provided in one set and if one of ECL is not sensitive enough, or there is too much background, the membrane can be washed and developed again, as described here. For laboratories without a CCD camera, visualization of the target protein can be made using so-called chromogenic detection with Agrisera BCIP/NBT ALP Substrate (AS19 BCIP-NBT) or Agrisera BCIP/NBT Plus ALP Substrate solution (AS19 BCIP-NBT-PLUS). In this case, the secondary antibody is conjugated to an enzyme called ALP (Alkaline Phosphatase). The reaction is visualized directly on the membrane through color development, and the membrane can be stored in the lab book for a future reference. This detection system is not appropriate for proteins of low expression, or to detect protein modifications, such as phosphorylation. | |
Latest
Agrisera Western blot workshop for North America2024-05-31 Agrisera supports 13th International Coference for Plant Mitochondrial Biology
2024-05-27 Study chloroplast biogenesis and archtecture with Agrisera antibodies
2024-05-24 Investigate proteins involved in sucrose metabolism with Agrisera antibodies
2024-05-19 Agrisera supports 9th Plant Biochemistry Symposium: Plant Metabolites and Signaling
2024-05-17 Track changes in LHC proteins using most published anti-LHC antibodies
2024-05-12 Agrisera supports BioLock student conference at the University of Wrocław
2024-05-10 Secondary Antibody Guide
2024-05-06 Shorten the time of Western blot or IL by using directly conjugated primary antibodies
2024-05-03 20 % off for antibodies to proteins involved in electron transfer in photosynthesis
2024-04-26
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