Anti-RPL33 | 50S ribosomal protein L33 (chloroplastic)

Product no: AS16 4082

AS16 4082 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
319
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated peptide, derived from Arabidopsis thaliana RPL33 UniProt: P56796 , TAIR: AtCg00640

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: for reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution:

    1: 2000 - 1: 5000 (WB)

    Expected | apparent MW: 7.6 | 10 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Zea mays
    Predicted reactivity:

    Acacia restiacea, Berberis bealei , Blossfeldia liliputana, Boehmeria spicata, Braya humilis, Calochortus albus, Citrullus colocynthis, Corymbia gummifera, Croomia heterosepala, Croton texensis, Cucurbita pepo, Dodonaea viscosa, Eucalyptus salmonophloia, Fragaria ananassa, Gossypium populifolium, Hippophae rhamnoides, Holodiscus discolor, Khaya senegalensis, Lessertia frutescens, Morus alba var. atropurpurea, Natsiatum herpeticum, Oryza sativa, Pachycladon cheesemanii , Pereskia sacharosa, Polylepis sp., Potentilla lancinata, Ranzania japonica, Ribes fasciculatum var. chinense, Rubus niveus, Solanum lycopersicum, Solanum tuburosum, Sonneratia alba, Trifolium semipilosum, Typha domingensis, Urophysa rockii, Vicia faba , Vicia sepium, Ximenia americana

    Species of your interest not listed? Contact us
    Not reactive in: Cyanobacteria
  • Application Examples
  • Application example

    10 µg of stromal protein from Arabidopsis thaliana (1) and Zea mays (2) denatured denatured  at 95°C 10 minutes were separated on  4-20% SDS-PAGE  gradient gel and blotted 1h to PVDF using wet transfer (80V 1 h 30 minutes). Blot was blocked with 5 % milk in TBS-T 1h/RT. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:20 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent and touch exposure was applied.

    Courtesy of Dr. Louis-Valentin Mereignier, CNRS, France

  • Additional Information
  • Additional information (application): Cross-reacting bands may appear when total cell extract is analysed.
  • Background
  • Background: RPL33 | 50S ribosomal protein L33 (chloroplastic) involved in translation process, large subunit of a ribosomal complex. Belongs to bacterial, ribosomal protein bl33 family.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS12 2115 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

416 €
Add to cart
Info

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info

AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

218 €
Add to cart
Info
Close