Anti-Clathrin heavy-chain 1,2, ALP-conjugated (40 ”g)

Product no: AS10 690-ALP

AS10 690-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
    Format: Liquid
    Quantity: 40 ”g
    Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
    Tested applications: Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: 1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
    Expected | apparent MW:

    193 | 170 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
    Predicted reactivity:

    Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandisGlycine max, Chlorella variabilisLeucaena glauca, Lotus japonicusMedicago tribuloidesMimulus guttatus, Musa malaccensisOryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera


    Species of your interest not listed? Contact us
    Not reactive in:

    Nicotiana benthamiana

  • Additional Information
  • Additional information (application): Please, note that fresh samples will provide better restuls (see image below)
  • Background
  • Background:

    Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.

     

  • Product Citations
  • Selected references: Gao et al. (2019). The Arabidopsis receptor kinase STRUBBELIG undergoes clathrin-dependent endocytosis. J Exp Bot. 2019 Apr 25. pii: erz190. doi: 10.1093/jxb/erz190.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS10 690  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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