Anti-PLDA1/2 | Phospholipase D alpha 1/2

Product no: AS12 2364

AS12 2364 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. oleracea, S. lycopersicum, L. luteus

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DATA SHEET IN PDF
Delivery:  3-6 business days
  • Data sheet
  •  
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PLD alpha 1, UniProt: Q38882,  TAIR: AT3G15730 and PLD alpha 2, UniProt: Q9SSQ9, TAIR: AT1G52570
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 91.8 (PLD alpha 1 precursor), 91.5 (PLD alpha 2 precursor)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea
    Predicted reactivity: Brassica napus, Brassica rapa, Capsella rubella, Carica papaya, Gossypium hirsutum, Glycine max, Ricinus communis

    Species of your interest not listed? Contact us
    Not reactive in: Olea europea, Zea mays
  • Background
  • Background: PLDA1/2 (phospholipase D alpha 1/2) is an enzyme which hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond and plays an important tole in processes as phytochormone action and response to stress. This protein is highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in seeds. Induced by ABA, ethylene, heavy metal, cold, salt and osmotic stresses. 

    Alternative names:PLDalpha1,PLD alpha 1,Choline phosphatase 1 PLDalpha, Phosphatidylcholine-hydrolyzing phospholipase D 1, Choline phosphatase 2,  Phosphatidylcholine-hydrolyzing phospholipase D 2, PLDalpha2, PLD alpha 2.
  • Product Citations
  • Selected references: Kocourkova et al. (2020). Phospholipase Da1 mediates the high-Mg2+ stress response partially through regulation of K+ homeostasis. Plant Cell Environ. 2020 Jun 25.doi: 10.1111/pce.13831.
  • Protocols



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