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Anti-ABA | Abscisic acid (C1) (1 mg)
AS09 422 | Clonality: Polyclonal | Host: Rabbit
- Product Info
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Immunogen: BSA-conjugated abscisic acid (C1) via C1 carboxyl group
Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein G purified in PBS. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 70 µl of dest, water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Radioimmunoassay (RIA) Recommended dilution: 2-10 µl/15 ml; Specific information about dilution is going to be included on the vial - Reactivity
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Confirmed reactivity: Abscisic acid (C1) Predicted reactivity: Abscisic acid (C1) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: ABA | Abscisic acid (C1) (1 mg) Additional information (application): For ELISA kit - please inquire - Background
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Background: Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.
- Product Citations
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Selected references: Domingues et al. (2024). Competitive Immunoassay in a Microfluidic Biochip for In-Field Detection of Abscisic Acid in Grapes. Biosensors (Basel). 2024 Feb 26;14(3):123. - Protocols
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Assay parameters of anti-abscisic acid (C1) antibodies in ELISA
Titre 1: 5300 Unspecific binding 2.8 % Midrange (B/Bo=50%) 275 pmol Detection limit 7.8 fmol, 2pg Linear range of measurment 15-5000 fmol Intraassay variability 4.3 % Intraassay variability 5.2 % Amount of tracer per assay 12.4 ng ELISA graph for anti-abscisic acid (C1) antibodies
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Molar cross-reactivities of different abscisic acids and related compunds with anti-abscisic acid (C1) antibodies
Data presented are expressed as percentage ratio of molar concentrations of appropriate ABA and competitor producing 50% inhibition in ELISA.
Compund Cross-reactivity (%) (±)-cis,trans-abscisic acid 100 (-)-cis,trans-abscisic acid 47 (+)-cis,trans-abscisic acid 52 (±)-trans,trans-abscisic acid 3.27 (+)-trans,trans-abscisic acid 1.06 (±)-cis,trans-abscisic acid methyl ester 217 (±)-cis,trans-abscisic acid glucosyl ester 115 phaseic acid 0 diphydrophaseic acid 0.09 xanthoxin 0.05 lunularic acid 0 violaxanthin- 0 farnesol 0 vomifoliol 0 Sample preparation
Processing of plant extracts required prior ELISA analysis may vary from plant to plant and will depend on the actual research objective. In most cases, the removal of pigemtns and lypophilic material by C18 reversed phase chromatography and subsequently by combined DEAE cellulose-reversed phase C18 is all that required. the ABA samples have to be methylated by diazomethane before ELISA analysis.
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