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Anti-Lhcbm5 | Chlorophyll a-b binding protein of LHCII
AS09 408 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
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Immunogen: SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 5000-1 : 10 000 (WB) Expected | apparent MW: 29 | 30 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Chlamydomonas reinhardtii
- Additional Information
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Additional information: This antibody cross-reacts with three major LHCII proteins of Chlamydomonas, which are slightly smaller than Lhcam5 on SDS-gel. 6M urea SDS-PAGE is one of the best systems that separate Lhcbm5 and the other major LHCII proteins. The dilution of the antibody should be carefully determined to reduce the cross-reactions with other major LHCII proteins, we recommend for this purpose to use the dilution of 1: 10 000- 1: 50 000
This product can be sold containing ProClin in requested.Additional information (application): For western blot detection image please refer to the article below - Background
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Background: Lhcbm5 is one of minor LHCII that associates with PSI in state-2
- Product Citations
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Selected references: Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.
Pinnola (2021). The rise and fall of Light-Harvesting Complex Stress-Related proteins as photoprotection agents during evolution. J Exp Bot. 2019 Oct 24;70(20):5527-5535. doi: 10.1093/jxb/erz317. PMID: 31424076.
Nama et al. (2018). Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.
Jeong et al. (2017). Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457.
Jeong et al. (2016). Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii. Biochim Biophys Acta. 2016 Oct 17;1858(1):45-55. doi: 10.1016/j.bbabio.2016.10.007.
Grewe et al. (2014). Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii. Plant Cell. 2014 Apr 4;26(4):1598-1611. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Accessories
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