Diatoms

Below you will find antibodies with confirmed or predicted reactivity to Diatoms.

Suggested Control Antibodies for Diatoms

AtpB | beta subunit of ATP synthase, AS05 085 | Clonality: polyclonal | Host: rabbit
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.

RbcL | Rubisco large subunit, form I, AS03 037 | Clonality: polyclonal | Host: rabbit
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.

PsbA | D1 protein of PSII, C-terminal, AS05 084 | Clonality: polyclonal | Host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using a sensitive chemiluminescent detection system like Agrisera ECLBright (extreme low femtogram range).

Protocol for protein extraction from diatoms

Protocols and Technical Information • Support • Western Blot Video Tutorials

Agrisera antibodies Diatoms

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1 | PEB (4x) | protein extraction buffer

AS08 300 | Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

119 €

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Anti-ACT | Actin (polyclonal)

AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays

319 €

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Anti-APX1 | Ascorbate peroxidase (algal)

AS15 2992 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

319 €

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Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken antibodies)

AS03 030 | Clonality: Polyclonal | Host: Chicken | Reactivity: [global antibody] for plant and bacterial F-type ATP synthases

Benefits of using this antibody

416 €

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Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

AS05 085 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for plant, green alga, animal and bacterial F-type ATP synthases

Benefits of using this antibody

319 €

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Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 �l)

AS05 085-10 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for plant, green alga and bacterial F-type ATP synthases

Benefits of using this antibody

175 €

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Anti-AtpB | Beta subunit of ATP synthase, mitochondrial

AS16 3976 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica oleracea var. botrytis cv. ’Diadom’, [compartment marker] of mitochondrial inner membrane

Benefits of using this antibody

319 €

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Anti-Cat | Catalase (algal)

AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Scenedesmus sp.

319 €

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Anti-Dsp | Death-specific protein

AS10 935 | Clonality: Polyclonal | Host: Rabbit | Reactivity: diatoms

409 €

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Anti-FCP | Fucoxanthin Chla/c protein

AS17 4116 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Heterosigma akashiwo

319 €

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Anti-FtsH1-11 | ATP-dependent zinc metalloprotease FtsH1-11

AS11 1789 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. acetabulum, A. thaliana, B. hypnoides (ulvophytes), G. theta, H. vulgare, Synechocystis sp., T. pseudonana, Z. mays

Benefits of using this antibody

319 €

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Anti-H+ATPase | Plasma membrane H+ATPase (chicken antibody)

AS13 2671 | Clonality: Polyclonal | Host: Chicken | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae cellular [compartment marker] for plasma membrane

Benefits of using this antibody

319 €

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Anti-H3 | Histone H3 (rabbit antibody) (nuclear marker)

AS10 710 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. cinerea, C. reinhardtii, H. vulgare, S. lycopersicum, V. faba, P. tricornutum, P. patens, S. europaea, Z. mays | cellular [compartment marker] of nucleoplasm

319 €

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Anti-Lhc1 | from PSI of red alga

AS08 282 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Diatoms

390 €

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Anti-LHCR4 | Protein fucoxanthin chlorophyll a/c protein

AS19 4303 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Phaeodactylum tricornutum

319 €

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Anti-Lhcx (P. tricornutum)

AS19 4367 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Pheodactyllum tricornutum

319 €

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Anti-Lhcx6 | Lhcx subclade of fucoxanthin Chl a/c proteins

AS17 4115 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Cyclotella sp., Thalassiosira pseudonana

319 €

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Anti-NifH | Nitrogenase iron protein

AS01 021A | Clonality: Polyclonal | Host: Hen | Reactivity: [global antibody] for bacteria

Benefits of using this antibody

416 €

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Anti-NR | Nitrate reductase, assimilatory

AS08 310 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, C. reinhardtii, red alga Gracilaria gracilis, Medicago sativa, diatom Thalassiosira sp., P. tricornutum Bohlin, P. yunnanensis Dode, P. notoginseng, S. lycopersicum, S. tuberosum

319 €

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Anti-Pectic polysaccharide, Xylogalacturonan (monoclonal, clone LM8)

AS18 4202-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

260 €

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Anti-PEPC | Phosphoenolpyruvate carboxylase

AS09 458 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, Ch. quinoa, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

Benefits of using this antibody

319 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, ALP-conjugated (40 �g)

AS09 458-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

Benefits of using this antibody

490 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, Biotin-conjugated (40 �g)

AS09 458B | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

Benefits of using this antibody

490 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, DyLight� 488 conjugated (40 �g)

AS09 458-DL488 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

490 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, DyLight� 594 conjugated (40 �g)

AS09 458-DL594 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

490 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, DyLight� 650 conjugated (40 �g)

AS09 458-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

490 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, HRP-conjugated (40 �g)

AS09 458-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

Benefits of using this antibody

490 €

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Anti-PetC | Rieske iron-sulfur protein of Cyt b6/f complex

AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. rapa subsp. chinensis, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, S. oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Z. mays

Benefits of using this antibody

319 €

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Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase

AS07 257 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, O. sativa, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

319 €

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Anti-PRK ribulose-5-P-kinase | phosphoribulokinase, ALP-conjugated (40 �g)

AS07 257-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

490 €

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