Anti-Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII

Altered protein accumulation and stability of the chloroplast ATP synthase in the cgl160 mutant visualized by immunoblotting.A. Immunoblots with antibodies against essential subunits of the photosynthetic protein complexes of wild-type (Col-4) Arabidopsis and the two cgl160 T-DNA insertion lines grown under long-day and short-day conditions. Isolated thylakoid membranes were used, and equal amounts of chlorophyll were loaded onto the SDS-PAGE gel. For approximate quantification, wild-type samples from long-day plants were diluted to 10%, 25% and 50%, respectively. Accumulation of PSII was probed with antibodies against PsbB and PSBO. Additionally, the PSBS protein involved in NPQ and the minor PSII antenna protein LHCB4 were probed. Accumulation of the cytochrome b6f complex was probed with antibodies against the essential subunits PetA (cytochrome f), PetB (cytochrome b6), and PETC (Rieske protein). Accumulation of PSI was probed with antibodies against the reaction center subunit PsaB and the stromal ridge subunit PsaD. ATP synthase accumulation was probed with antibodies against the CF1 subunits AtpA (CF1?), AtpB (CF1?) and AtpD (CF1?) and antibodies against the CF0 subunits AtpF (CF0b) and AtpI (CF0a). B. Loading difference estimation for immunoblotting CF1 between wild type and cgl160-1. To obtain similar immunoblotting signal three times more (15 ?g protein) was needed for cgl160-1 compared to wild type (5 ?g protein). C. Maintenance of CF1 was measured by incubating leaves from wild type and cgl160-1 in solution containing the plastid protein synthesis inhibitor chloramphenicol for the indicated time points. Protein extract was isolated and separated by SDS-PAGE, immunoblotted and probed with specific antibodies against CF1 and LHCB2.1. Three times more protein was loaded from the mutant to obtain equal level of CF1 immunoblotting signal, as specified in B.

Light- and 1O2-dependent EX1 degradation. Continuous light (CL)-grown 5-day-(d)-old transgenic seedlings of aex1 and bex1 flu expressing EX1-GFP under the control of the 35S promoter were transferred to the dark (for 2, 4, 8?h) and the 8-h-dark (D)-treated seedlings were re-exposed to light (for 0.5, 1, 2?h) at the light intensity of 100?µmolm-2?s-1. Total protein was extracted and analyzed by western blot. Chlorophyll a/b binding protein CP29 (Lhcb4) and cytosolic UDP-glucose pyrophosphorylase (UGP) were used as controls. EX1-GFP, Lhcb4, and UGPase were detected using antibodies against GFP, Lhcb4, and UGP, respectively. c The levels of EX1-GFP in the dark or after re-exposing to light were compared to its abundances under CL conditions. Average intensity values of the protein bands were calculated using AzureSpot software v14.0 (AZURE). Data represents the mean of three biological repeats. Error bars show standard error of the mean. Asterisks in c indicate statistically significant differences to CL condition (P?<?0.05, Student’s t-test)